Biosynthesis and Characterization of Silver Nanoparticles from Cassia Auriculata Leaf Extract and in Vitro Evaluation of Antimicrobial Activity

The synthesis of metals and nanoparticles is an expanding research area due to the potential applications for the development of novel technologies. Biosynthesis of silver nanoparticles was investigated by reducing sliver nitrate with Cassiaauriculataleaf extract at room temperature. The plant belongs to family Ceasalpiniaceae and the plant is having promising medicinal properties for a wide range of human diseases. The synthesized nanoparticles characterized by the UV-Vis spectroscopy, revealed the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 420-435 nm.The peaks in the X ray Diffraction pattern are in good agreement with the standard values of the face-centered-cubic form of metallic silver. Fourier transform infrared spectroscopy indicates that the compounds attached with silver nanoparticles could be polyphenols with aromatic ring and bound amide region and transmission electron microscope reveals that the particles are spherical and polydispersed.The antimicrobial activity of synthesized nanoparticles were evaluated against E.coli, Sarratiamarcascence, Bacillus subtilis ,Aspergillusniger and Aspergillusflavus. Fungi were most susceptible to silver nanoparticles followed by bacteria.